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The effect of nitric oxide on adherence of P-fimbriated uropathogenic Escherichia coli to human renal epithelial cells
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. Ecology and Evolution in Microbial Model Systems, EEMiS. (Marine Microbial Ecology)
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
2010 (English)In: British Journal of Urology, ISSN 0007-1331, E-ISSN 1365-2176, Vol. 105, no 12, 1726-1731 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES

To examine the effect of nitric oxide (NO), an endogenous component of the host defence in urinary tract infection, on the adherence of P-fimbriated uropathogenic Escherichia coli (UPEC) to human renal epithelial cells.

MATERIALS AND METHODS

Two wild-type UPEC strains (AD110 and IA2) and the P-fimbriated recombinant strain HB101pPIL-75 were used. Bacteria were allowed to adhere to the human renal epithelial cell line A498 and attachment was evaluated in the absence or presence of the NO donor DETA/NONOate (1 mm). Total RNA was extracted from NO-exposed bacteria in static urine cultures, followed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of the papG gene that encodes the P-fimbriae adhesin PapG.

RESULTS

Bacterial adherence to A498 cells was fimbriae-dependent and the ability to agglutinate human P1 positive erythrocytes confirmed that the used strains were P-fimbriated. UPEC strains AD110 and IA2 attached by a mean of 8 bacteria/cell and 20 bacteria/cell, respectively. In the presence of DETA/NONOate, the attachment of AD110 and IA2 to A498 cells was significantly reduced by a mean (sem) of 34 (3.9)% and 45 (14)%, respectively. The expression of papG was decreased after DETA/NONOate exposure as shown by semiquantitative RT-PCR.

CONCLUSION

NO disrupted functional adhesion of P-fimbriated UPEC to kidney epithelial cells, suggesting that NO-production from epithelial cells in the urinary tract may limit bacterial colonization at the mucosal surface. The reduced adherence may involve transcriptional effects of NO on papG expression, but further studies are needed to establish the underlying mechanisms.

Place, publisher, year, edition, pages
2010. Vol. 105, no 12, 1726-1731 p.
National Category
Microbiology in the medical area
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-29101DOI: 10.1111/j.1464-410X.2009.08986.xOAI: oai:DiVA.org:lnu-29101DiVA: diva2:652310
Available from: 2013-09-30 Created: 2013-09-30 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Nitric oxide and bacteria-host interactions in Escherichia coli urinary tract infection
Open this publication in new window or tab >>Nitric oxide and bacteria-host interactions in Escherichia coli urinary tract infection
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Kalmar: Högskolan i Kalmar, 2008. 53 p.
Series
Dissertation series / University of Kalmar, Faculty of Natural Science, ISSN 1650-2779 ; 51
National Category
Microbiology in the medical area
Research subject
Biomedical Sciences, Pharmacology
Identifiers
urn:nbn:se:lnu:diva-29096 (URN)978-91-85993-01-7 (ISBN)
Public defence
2008-04-25, Västergårds hörsal, Smålandsgatan 26b, Kalmar, 10:00
Opponent
Supervisors
Available from: 2013-11-20 Created: 2013-09-30 Last updated: 2017-01-24Bibliographically approved

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Svensson, LovisaSäve, SusannePersson, Katarina

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