lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli – implications for urinary tract infection
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences. (Marine Microbial Ecology;Ctr Ecol & Evolut Microbial Model Syst EEMiS)
Karolinska Institutet.
Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
University of Sheffield, UK.
Show others and affiliations
2010 (English)In: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 49, no 3, p. 59-66Article in journal (Refereed) Published
Abstract [en]

During the course of urinary tract infection (UTI) nitric oxide (NO) is generated as part of the host response. This study investigates the significance of the NO-detoxifying enzyme flavohemoglobin (Hmp) in protection of uropathogenic Escherichia coli (UPEC) against nitrosative stress. An hmp (J96Δhmp) knockout mutant of UPEC strain J96 was constructed using single-gene deletion. The viability of J96Δhmp was significantly reduced (P < 0.001) compared to the wild-type strain after exposure to the NO-donor DETA/NO. The NO consumption in J96Δhmp was significantly (P < 0.001) impaired compared to J96wt. Screening UPEC isolates from patients with UTI revealed increased hmp expression in all patients. In a competition-based mouse model of UTI, the hmp mutant strain was significantly (P < 0.05) out-competed by the wild-type strain. This study demonstrates, for the first time, that Hmp contributes to the protection of UPEC against NO-mediated toxicity in vitro. In addition, hmp gene expression occurs in UPEC isolates from the infected human urinary tract and UPEC that were hmp-deficient had a reduced ability to colonize the mouse urinary tract. Taken together the results suggest that NO detoxification by Hmp may be a fitness advantage factor in UPEC, and a potentially interesting target for development of novel treatment concepts for UTI.

Place, publisher, year, edition, pages
2010. Vol. 49, no 3, p. 59-66
National Category
Microbiology in the medical area
Research subject
Natural Science, Biomedical Sciences
Identifiers
URN: urn:nbn:se:lnu:diva-29102DOI: 10.1016/j.micpath.2010.04.001Scopus ID: 2-s2.0-77954660611OAI: oai:DiVA.org:lnu-29102DiVA, id: diva2:652312
Note

Ingår i avhandlingen "Nitric oxide and bacteria-host interactions in Escherichia coli urinary tract infection" under titeln "Flavohemoglobin protects uropathogenic Escherichia coli against nitrosative stress; implication for urovirulence"

Available from: 2013-09-30 Created: 2013-09-30 Last updated: 2018-10-24Bibliographically approved
In thesis
1. Nitric oxide and bacteria-host interactions in Escherichia coli urinary tract infection
Open this publication in new window or tab >>Nitric oxide and bacteria-host interactions in Escherichia coli urinary tract infection
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Kalmar: Högskolan i Kalmar, 2008. p. 53
Series
Dissertation series / University of Kalmar, Faculty of Natural Science, ISSN 1650-2779 ; 51
National Category
Microbiology in the medical area
Research subject
Biomedical Sciences, Pharmacology
Identifiers
urn:nbn:se:lnu:diva-29096 (URN)978-91-85993-01-7 (ISBN)
Public defence
2008-04-25, Västergårds hörsal, Smålandsgatan 26b, Kalmar, 10:00
Opponent
Supervisors
Available from: 2013-11-20 Created: 2013-09-30 Last updated: 2018-01-11Bibliographically approved

Open Access in DiVA

Fulltext(577 kB)218 downloads
File information
File name FULLTEXT01.pdfFile size 577 kBChecksum SHA-512
2484df54c65d6781127f724d0a8f8c592477a14d853d46528c52abcba7d4ea4eae94550af493baba6db6bd621211a8bc2d8f4bc55922ce83853eebae0b1e59f0
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Authority records BETA

Svensson, LovisaSäve, SusannePersson, Katarina

Search in DiVA

By author/editor
Svensson, LovisaSäve, SusannePersson, Katarina
By organisation
School of Natural Sciences
In the same journal
Microbial Pathogenesis
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 218 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 131 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf