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Effects of overexpression of AaWRKY1 on artemisinin biosynthesis in transgenic Artemisia annua plants
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Zhejiang Agriculture and Forestry University, Linan 311300, Zhejiang, PR China..
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.ORCID iD: 0000-0001-8899-5046
2014 (English)In: Phytochemistry, ISSN 0031-9422, E-ISSN 1873-3700, Vol. 102, p. 89-96Article in journal (Refereed) Published
Abstract [en]

The effective anti-malarial medicine artemisinin is costly because of the low content in Artemisia annua. Genetic engineering of A. annua is one of the most promising approaches to improve the yield of artemisinin. In this work, the transcription factor AaWRKY1, which is thought to be involved in the regulation of artemisinin biosynthesis, was cloned from A. annua var. Chongqing and overexpressed using the CaMV35S promoter or the trichome-specific CYP71AV1 promoter in stably transformed A. annua plants. The transcript level of AaWRKY1 was increased more than one hundred times under the CaMV35S promoter and about 40 times under the CYP71AV1 promoter. The overexpressed AaWRKY1 activated the transcription of CYP71AV1 and moreover the trichome-specific overexpression of AaWRKY1 improved the transcription of CYP71AV1 much more effectively than the constitutive overexpression of AaWRKY1, i.e. up to 33 times as compared to the wild-type plant. However the transcription levels of FDS, ADS, and DBR2 did not change significantly in transgenic plants. The significantly up-regulated CYP71AV1 promoted artemisinin biosynthesis, i.e. up to about 1.8 times as compared to the wild-type plant. It is demonstrated that trichome-specific overexpression of AaWRKY1 can significantly activate the transcription of CYP71AV1 and the up-regulated CYP71AV1 promotes artemisinin biosynthesis.

Place, publisher, year, edition, pages
2014. Vol. 102, p. 89-96
Keywords [en]
AaWRKY1; Artemisia annua; Artemisinin biosynthesis; CYP71AV1 promoter; CYP71AV1; Trichome-specific overexpression
National Category
Biochemistry and Molecular Biology
Research subject
Natural Science, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-33362DOI: 10.1016/j.phytochem.2014.02.011ISI: 000336339700008Scopus ID: 2-s2.0-84899438852OAI: oai:DiVA.org:lnu-33362DiVA, id: diva2:708459
Available from: 2014-03-27 Created: 2014-03-27 Last updated: 2017-12-05Bibliographically approved

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Han, JunliWang, HongzhenLundgren, AnneliBrodelius, Peter E.

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