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Comparison of weak affinity chromatography and surface plasmon resonance in determining affinity of small molecules
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
Linköping Univ, Sweden.
Linköping Univ, Sweden.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
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2014 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 461, 57-59 p.Article in journal (Refereed) Published
Abstract [en]

In this study, we compared affinity data from surface plasmon resonance (SPR) and weak affinity chromatography (WAC), two established techniques for determination of weak affinity (mM-mu M) small molecule-protein interactions. In the current comparison, thrombin was used as target protein. In WAC the affinity constant (K-D) was determined from retention times, and in SPR it was determined by Langmuir isotherm fitting of steady-state responses. Results indicate a strong correlation between the two methods (R-2 = 0.995, P < 0.0001). (C) 2014 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2014. Vol. 461, 57-59 p.
Keyword [en]
Affinity determination, Fragment-based drug discovery, Surface plasmon resonance, Thrombin, Weak affinity chromatography
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-36862DOI: 10.1016/j.ab.2014.05.023ISI: 000340077600009OAI: oai:DiVA.org:lnu-36862DiVA: diva2:745576
Available from: 2014-09-10 Created: 2014-09-10 Last updated: 2016-12-20Bibliographically approved

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Duong-Thi, Minh-DaoBergström, MariaOhlson, Sten
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