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Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1.
University of East Anglia, UK ; University of Georgia, USA.
University of East Anglia, UK ; Umeå University.ORCID iD: 0000-0002-9622-3318
University of East Anglia, UK.
John Innes Centre, Norwich, UK.
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2007 (English)In: Extremophiles, ISSN 1431-0651, E-ISSN 1433-4909, Vol. 11, no 3, 425-34 p.Article in journal (Refereed) Published
Abstract [en]

'Ferroplasma acidarmanus' Fer1 is an arsenic-hypertolerant acidophilic archaeon isolated from the Iron Mountain mine, California; a site characterized by heavy metals contamination. The presence of up to 10 g arsenate per litre [As(V); 133 mM] did not significantly reduce growth yields, whereas between 5 and 10 g arsenite per litre [As(III); 67-133 mM] significantly reduced the yield. Previous bioinformatic analysis indicates that 'F. acidarmanus' Fer1 has only two predicted genes involved in arsenic resistance and lacks a recognizable gene for an arsenate reductase. Biochemical analysis suggests that 'F. acidarmanus' Fer1 does not reduce arsenate indicating that 'F. acidarmanus' Fer1 has an alternative resistance mechanism to arsenate other than reduction to arsenite and efflux. Primer extension analysis of the putative ars transcriptional regulator (arsR) and efflux pump (arsB) demonstrated that these genes are co-transcribed, and expressed in response to arsenite, but not arsenate. Two-dimensional polyacrylamide gel electrophoresis analysis of 'F. acidarmanus' Fer1 cells exposed to arsenite revealed enhanced expression of proteins associated with protein refolding, including the thermosome Group II HSP60 family chaperonin and HSP70 DnaK type heat shock proteins. This report represents the first molecular and proteomic study of arsenic resistance in an acidophilic archaeon.

Place, publisher, year, edition, pages
2007. Vol. 11, no 3, 425-34 p.
National Category
Microbiology
Research subject
Natural Science, Microbiology
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URN: urn:nbn:se:lnu:diva-37330DOI: 10.1007/s00792-006-0052-zPubMedID: 17268768OAI: oai:DiVA.org:lnu-37330DiVA: diva2:750364
Available from: 2014-09-29 Created: 2014-09-29 Last updated: 2016-11-30Bibliographically approved

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CiteExportLink to record
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Cite
Citation style
  • apa
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  • modern-language-association-8th-edition
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