lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Quantification of alkali-labile phosphate groups in the plasma of Oreochromis niloticus exposed to intermittent discharges of oestrogens: effect of concentration vs. load
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Rio de Janeiro State Univ UERJ, Rio De Janeiro, Brazil.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science. Rio de Janeiro State Univ UERJ, Rio De Janeiro, Brazil.ORCID iD: 0000-0002-7920-8001
2014 (English)In: International Journal of Environmental Analytical Chemistry, ISSN 0306-7319, E-ISSN 1029-0397, Vol. 94, no 11, 1161-1172 p.Article in journal (Refereed) Published
Abstract [en]

Vitellogenin protein (Vtg) in Oreochromis niloticus plasma has been indirectly quantified through protein-bound phosphate groups also known as alkali-labile phosphates (ALP) using a recently modified method. Such method as described in the literature was originally applied to Crucian carp and resulted in lower detection limits (3.2 mu g PO(4)(3-)per mL). In this study, O. niloticus males were exposed to intermittent doses of oestrogens for 15 days using different concentrations (converted to loads) of 17 alpha-ethinyloestradiol (EE2) (two different aquarium volumes), oestrone (E1) and 17 beta-oestradiol (E2) individually and in combination (1:1:1). The induction of physiologic and genotoxic effects in erythrocytes was investigated. For the tested oestrogen (EE2), load proved to be more relevant than concentration in determining the oestrogenicity. O. niloticus males proved to have lower ALP baseline (4.11 mu g PO43-/mL plasma, IQ(25)=3.38; IQ(75)=5.18) than other fish species, including Crucian carp, which makes it suitable for oestrogenicity detection in water. Exposure to E2, EE2 separately and in combination (1:1:1) all induced significant increases in the ALP levels at loads >= 0.72 mu g/fish. This load was three times lower than the E1 load required to increase ALP (>= 2.2 mu g/fish). All oestrogens with loads >= 0.072 mu g/fish caused significant increase in micronucleus frequency (>= 2 parts per thousand) compared with the control (0.1 +/- 0.4 parts per thousand). The study highlighted the importance of taking into account not only concentration and dose regime but also the mass load and therefore, the volume used in the experimental units, which is rarely addressed in ecotoxicity assays. Considering the good sensitivity of O. niloticus exposed to relatively low concentrations of oestrogens, the combination of the ALP method with auxiliary biomarkers (particularly micronucleus) can be used as a protocol for oestrogenicity and genotoxicity detection in different contaminated waters as part of water environmental monitoring programmes.

Place, publisher, year, edition, pages
2014. Vol. 94, no 11, 1161-1172 p.
Keyword [en]
intermittent discharge, Oreochromis niloticus, micronucleus frequency, alkali-labile phosphates, oestrogen load
National Category
Environmental Sciences
Research subject
Environmental Science, Environmental Chemistry
Identifiers
URN: urn:nbn:se:lnu:diva-38314DOI: 10.1080/03067319.2014.930845ISI: 000343609100008OAI: oai:DiVA.org:lnu-38314DiVA: diva2:765644
Available from: 2014-11-24 Created: 2014-11-24 Last updated: 2017-03-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
de Sa Salomao, Andre LuisMarques, Marcia
By organisation
Department of Biology and Environmental Science
In the same journal
International Journal of Environmental Analytical Chemistry
Environmental Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 52 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf