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Evaluation of High-Resolution Melting Curve Analysis of Ligation-Mediated Real-Time PCR, a Rapid Method for Epidemiological Typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) Pathogens
Linnaeus University, Faculty of Health and Life Sciences, Department of Medicine and Optometry.ORCID iD: 0000-0002-1707-2655
Linköping Univ.
Publ Hlth Agcy Sweden, Solna, Sweden.
Linköping Univ.
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2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 12, 4339-4342 p.Article in journal (Refereed) Published
Abstract [en]

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.

Place, publisher, year, edition, pages
2014. Vol. 52, no 12, 4339-4342 p.
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-39151DOI: 10.1128/JCM.02537-14ISI: 000345222900033OAI: oai:DiVA.org:lnu-39151DiVA: diva2:781138
Available from: 2015-01-15 Created: 2015-01-15 Last updated: 2017-02-16Bibliographically approved

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Woksepp, HannaMarklund, Britt-Inger
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Department of Medicine and OptometryDepartment of Chemistry and Biomedical Sciences
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CiteExportLink to record
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