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Evaluation of High-Resolution Melting Curve Analysis of Ligation-Mediated Real-Time PCR, a Rapid Method for Epidemiological Typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) Pathogens
Linnaeus University, Faculty of Health and Life Sciences, Department of Medicine and Optometry.ORCID iD: 0000-0002-1707-2655
Linköping University.
Publ Hlth Agcy Sweden, Solna, Sweden.
Linköping University.
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2014 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 52, no 12, p. 4339-4342Article in journal (Refereed) Published
Abstract [en]

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.

Place, publisher, year, edition, pages
2014. Vol. 52, no 12, p. 4339-4342
National Category
Microbiology
Research subject
Ecology, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-39151DOI: 10.1128/JCM.02537-14ISI: 000345222900033Scopus ID: 2-s2.0-84910147917OAI: oai:DiVA.org:lnu-39151DiVA, id: diva2:781138
Available from: 2015-01-15 Created: 2015-01-15 Last updated: 2017-11-01Bibliographically approved

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Woksepp, HannaMarklund, Britt-Inger

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Department of Medicine and OptometryDepartment of Chemistry and Biomedical Sciences
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