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Cellular nutrient content measured with the nuclear microprobe and toxins produced by Dinophysis norvegica (Dinophyceae) from the Trondheim fjord (Norway)
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.
Lund University.
Tama Lab, Japan.
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2015 (English)In: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 75, no 3, p. 259-269Article in journal (Refereed) Published
Abstract [en]

Dinophysis norvegica is a commonly occurring dinoflagellate species and a producer of diarrhetic shellfish poisons. Natural samples were collected from Trondheim fjord, Norway, to analyse nutrient (O, C, N, P) and toxin (dinophysitoxins [DXTs], okadaic acid [OA], pectenotoxins [PTXs]) content in D. norvegica cells. Nutrient and toxin analyses were also carried out on cells grown under nutrient-sufficient and nutrient-deficient conditions to determine how intracellular nutrient and toxin content varied under different nutrient availability conditions. Nutrient analyses were conducted using nuclear microprobe techniques that can accurately analyse single cells, and toxin analyses were carried out using liquid chromatography and mass spectroscopy. The intracellular carbon, nitrogen and phosphorus content in individual cells varied greatly, and intracellular C:N:P ratios showed that the cells were both N- and P-deficient when compared to the Redfield ratio. The ideal N:P ratio in the media for D. norvegica was found to be below the Redfield ratio, but intracellular ratios did not show a clear relationship with those in the media. N:P ratios of D. norvegica were higher than expected, which is likely due to their phagotrophy on zooplankon. The highest toxin values found were traces of PTX2, 24.72 pg PTX2SA cell(-1), 2.19 pg DTX1 cell(-1), and 1.01 pg OA cell(-1). However, we found no clear relationship between the content of intracellular nutrients and toxins.

Place, publisher, year, edition, pages
2015. Vol. 75, no 3, p. 259-269
Keywords [en]
Dinophysis, Diarrhetic shellfish poisoning, Elemental cellular content, Mixotrophy, Nuclear microprobe, Nutrient ratios, Nutrients, Trondheim fjord
National Category
Biological Sciences
Research subject
Ecology, Aquatic Ecology
Identifiers
URN: urn:nbn:se:lnu:diva-45825DOI: 10.3354/ame01762ISI: 000358512400006Scopus ID: 2-s2.0-84937910336OAI: oai:DiVA.org:lnu-45825DiVA, id: diva2:847938
Available from: 2015-08-21 Created: 2015-08-21 Last updated: 2017-12-07Bibliographically approved

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Pérez Blanco, EvaGranéli, Edna

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