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Expression and activity of the Calvin-Benson-Bassham cycle transcriptional regulator CbbR from Acidithiobacillus ferrooxidans in Ralstonia eutropha
Univ Antofagasta, Chile.
Fdn Ciencia & Vida, Chile.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.ORCID iD: 0000-0002-9622-3318
Fdn Ciencia & Vida, Chile ; Univ Andres Bello, Chile.
2015 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 362, no 15, UNSP fnv108Article in journal (Refereed) Published
Abstract [en]

Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene potentially encoding CbbR (cbbR(AF)) has been predicted in the genome of the chemolithoautotrophic, extreme acidophile Acidithiobacillus ferrooxidans. However, this microorganism is recalcitrant to genetic manipulation impeding the experimental validation of bioinformatic predictions. Two novel functional assays were devised to advance our understanding of cbbR(AF) function using the mutated facultative autotroph Ralstonia eutropha H14 Delta cbbR as a surrogate host to test gene function: (i) cbbR(AF) was expressed in R. eutropha and was able to complement Delta cbbR; and (ii) CbbR(AF) was able to regulate the in vivo activity of four A. ferrooxidans cbb operon promoters in R. eutropha. These results open up the use of R. eutropha as a surrogate host to explore cbbR(AF) activity.

Place, publisher, year, edition, pages
2015. Vol. 362, no 15, UNSP fnv108
Keyword [en]
Acidithiobacillus ferrooxidans, Ralstonia eutropha, autotroph, CbbR, RubisCO, CO2 fixation
National Category
Microbiology
Research subject
Natural Science, Microbiology
Identifiers
URN: urn:nbn:se:lnu:diva-46089DOI: 10.1093/femsle/fnv108ISI: 000359823500001OAI: oai:DiVA.org:lnu-46089DiVA: diva2:851434
Available from: 2015-09-04 Created: 2015-09-04 Last updated: 2016-11-30Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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