lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Sensing protein antigen and microvesicle analytes using high-capacity biopolymer nano-carriers
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
University of Padova, Italy.
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
University of Padova, Italy.
Show others and affiliations
2016 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 141, no 3, p. 836-846Article in journal (Refereed) Published
Abstract [en]

Lab-on-a-chip systems with molecular motor driven transport of analytes attached to cytoskeletal filament shuttles (actin filaments, microtubules) circumvent challenges with nanoscale liquid transport. However, the filaments have limited cargo-carrying capacity and limitations either in transportation speed (microtubules) or control over motility direction (actin). To overcome these constraints we here report incorporation of covalently attached antibodies into self-propelled actin bundles (nanocarriers) formed by cross-linking antibody conjugated actin filaments viafascin, a natural actin-bundling protein. We demonstrate high maximum antigen binding activity and propulsion by surface adsorbed myosin motors. Analyte transport capacity is tested using both protein antigens and microvesicles, a novel class of diagnostic markers. Increased incubation concentration with protein antigen in the 0.1–100 nM range (1 min) reduces the fraction of motile bundles and their velocity but maximum transportation capacity of >1 antigen per nm of bundle length is feasible. At sub-nanomolar protein analyte concentration, motility is very well preserved opening for orders of magnitude improved limit of detection using motor driven concentration on nanoscale sensors. Microvesicle-complexing to monoclonal antibodies on the nanocarriers compromises motility but nanocarrier aggregation via microvesicles shows unique potential in label-free detection with the aggregates themselves as non-toxic reporter elements.

Place, publisher, year, edition, pages
2016. Vol. 141, no 3, p. 836-846
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-48641DOI: 10.1039/C5AN02377GISI: 000368942600011Scopus ID: 2-s2.0-84956748686OAI: oai:DiVA.org:lnu-48641DiVA, id: diva2:892371
Funder
Carl Tryggers foundation EU, FP7, Seventh Framework ProgrammeAvailable from: 2016-01-10 Created: 2016-01-10 Last updated: 2018-09-04Bibliographically approved

Open Access in DiVA

fulltext(948 kB)1 downloads
File information
File name FULLTEXT01.pdfFile size 948 kBChecksum SHA-512
85d256478dc23d786325f4f09390cf4798a017289a81ff27cd3047d615a72619d93a798e57ffbfad79d9ce958dca6889773c5af76ab50c5c671c331ae95d08a9
Type fulltextMimetype application/pdf

Other links

Publisher's full textScopus

Authority records BETA

Kumar, SarojTakatsuki, HideyoPersson, MalinMånsson, Alf

Search in DiVA

By author/editor
Kumar, SarojTakatsuki, HideyoPersson, MalinMånsson, Alf
By organisation
Department of Chemistry and Biomedical Sciences
In the same journal
The Analyst
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 1 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 113 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf