lnu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Quantity of the dinoflagellate sxtA4 gene and cell density correlates with paralytic shellfish toxin production in Alexandrium ostenfeldii blooms
Univ Turku, Finland.
Univ Helsinki, Finland.
Åbo Akad Univ, Finland.
Linnaeus University, Faculty of Health and Life Sciences, Department of Biology and Environmental Science.
Show others and affiliations
2016 (English)In: Harmful Algae, ISSN 1568-9883, E-ISSN 1878-1470, Vol. 52, 1-10 p.Article in journal (Refereed) Published
Resource type
Text
Abstract [en]

Many marine dinofiagellates, including several species of the genus Alexandrium, Gymnodinium catenatum, and Pyrodinium bahamense are known for their capability to produce paralytic shellfish toxins (PST), which can cause severe, most often food-related poisoning. The recent discovery of the first PST biosynthesis genes has laid the foundation for the development of molecular detection methods for monitoring and study of PST-producing dinofiagellates. In this study, a probe-based qPCR method for the detection and quantification of the sxtA4 gene present in Alexandrium spp. and Gymnodinium catenatum was designed. The focus was on Alexandrium ostenfeldii, a species which recurrently forms dense toxic blooms in areas within the Baltic Sea. A consistent, positive correlation between the presence of sxtA4 and PST biosynthesis was observed, and the species was found to maintain PST production with an average of 6 genomic copies of sxtA4. In August 2014, A. ostenfeldii populations were studied for cell densities, PST production, as well as sxtA4 and species-specific LSU copy numbers in Foglo, Aland, Finland, where an exceptionally dense bloom, consisting of 6.3 x 10(6) cells L-1, was observed. Cell concentrations, and copy numbers of both of the target genes were positively correlated with total STX, GTX2, and GTX3 concentrations in the environment, the cell density predicting toxin concentrations with the best accuracy (Spearman's p = 0.93, p < 0.01). The results indicated that all A. ostenfeldii cells in the blooms harbored the genetic capability of PST production, making the detection of sxtA4 a good indicator of toxicity. (C) 2015 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2016. Vol. 52, 1-10 p.
Keyword [en]
Dinoflagellate, qPCR, paralytic shellfish toxin, Alexandrium, sxtA4
National Category
Ecology
Research subject
Ecology, Aquatic Ecology
Identifiers
URN: urn:nbn:se:lnu:diva-51118DOI: 10.1016/j.hal.2015.10.018ISI: 000370088300001OAI: oai:DiVA.org:lnu-51118DiVA: diva2:913379
Available from: 2016-03-21 Created: 2016-03-21 Last updated: 2016-03-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Lindehoff, Elin
By organisation
Department of Biology and Environmental Science
In the same journal
Harmful Algae
Ecology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 318 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf