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Quantification of isotope-labelled and unlabelled folates in plasma, ileostomy and food samples.
Technische Universität München, Germany.
Swedish University of Agricultural Sciences, Uppsala.
Swedish University of Agricultural Sciences, Uppsala.ORCID iD: 0000-0003-0387-4312
Technische Universität München, Germany.
2011 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 399, no 1, 429-439 p.Article in journal (Refereed) Published
Abstract [en]

New stable isotope dilution assays were developed for the simultaneous quantitation of [(13)C(5)]-labelled and unlabelled 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, folic acid along with unlabelled tetrahydrofolic acid and 10-formylfolic acid in clinical samples deriving from human bioavailability studies, i.e. plasma, ileostomy samples, and food. The methods were based on clean-up by strong anion exchange followed by LC-MS/MS detection. Deuterated analogues of the folates were applied as the internal standards in the stable isotope dilution assays. Assay sensitivity was sufficient to detect all relevant folates in the respective samples as their limits of detection were below 0.62 nmol/L in plasma and below 0.73 μg/100 g in food or ileostomy samples. Quantification of the [(13)C(5)]-label in clinical samples offers the possibility to differentiate between folate from endogenous body pools and the administered dose when executing bioavailability trials.

Place, publisher, year, edition, pages
2011. Vol. 399, no 1, 429-439 p.
National Category
Food Science
Research subject
Natural Science
Identifiers
URN: urn:nbn:se:lnu:diva-51144DOI: 10.1007/s00216-010-4313-4PubMedID: 21052651OAI: oai:DiVA.org:lnu-51144DiVA: diva2:913528
Available from: 2016-03-21 Created: 2016-03-21 Last updated: 2016-05-13Bibliographically approved

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