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The Bacterial (Vibrio alginolyticus) Production of Tetrodotoxin in the Ribbon Worm Lineus longissimus: Just a False Positive?
Swedish University of Agricultural Sciences ; Uppsala University. (Sven Lovén Centre for Marine Sciences)ORCID iD: 0000-0002-8144-9592
Lund University. (Department of Biotechnology)
University of Gothenburg. (Department of Biology and Environmental Sciences)
Liverpool John Moores University, UK.
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2016 (English)In: Marine Drugs, ISSN 1660-3397, E-ISSN 1660-3397, Vol. 14, no 4, article id 63Article in journal (Refereed) Published
Abstract [en]

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel–dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound <5 kDa, and via a different mechanism of action than that of TTX. The similarity in mass between TTX and the Vibrio-produced low-molecular-weight, non-toxic compound invokes that thorough analysis is required when assessing TTX production. Based on our findings, we suggest that re-examination of some published claims of TTX production may be warranted.

Place, publisher, year, edition, pages
Basel: MDPI , 2016. Vol. 14, no 4, article id 63
Keywords [en]
LC-MS; mucus; axonal conductance; Vibrio; nemertean; tetrodotoxin
Keywords [sv]
slemmaskar, nemertiner
National Category
Analytical Chemistry
Research subject
Chemistry, Medical Chemistry
Identifiers
URN: urn:nbn:se:lnu:diva-51493DOI: 10.3390/md14040063ISI: 000374588000002Scopus ID: 2-s2.0-84962751120OAI: oai:DiVA.org:lnu-51493DiVA, id: diva2:915056
Available from: 2016-03-29 Created: 2016-03-29 Last updated: 2017-12-07Bibliographically approved

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Strand, MalinGöransson, UlfAndersson, Håkan S.

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