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Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua
Wageningen University, The Netherlands.
Wageningen University, The Netherlands.
Université catholique de Louvain, Belgium.
Wageningen University, The Netherlands.
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2016 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 38, 159-169 p.Article in journal (Refereed) Published
Abstract [en]

Our lack of full understanding of transport and sequestration of the heterologous products currently limit metabolic engineering in plants for the production of high value terpenes. For instance, although all genes of the artemisinin/arteannuin B (AN/AB) biosynthesis pathway (AN-PW) from Artemisia annua have been identified, ectopic expression of these genes in Nicotiana benthamiana yielded mostly glycosylated pathway intermediates and only very little free (dihydro)artemisinic acid [(DH)AA]. Here we demonstrate that Lipid Transfer Protein 3 (AaLTP3) and the transporter Pleiotropic Drug Resistance 2 (AaPDR2) from A. annua enhance accumulation of (DH)AA in the apoplast of N. benthamiana leaves. Analysis of apoplast and cell content and apoplast exclusion assays show that AaLTP3 and AaPDR2 prevent reflux of (DH)AA from the apoplast back into the cells and enhances overall flux through the pathway. Moreover, AaLTP3 is stabilized in the presence of AN-PW activity and co-expression of AN-PW+AaLTP3+AaPDR2 genes yielded AN and AB in necrotic N. benthamiana leaves at 13 days post-agroinfiltration. This newly discovered function of LTPs opens up new possibilities for the engineering of biosynthesis pathways of high value terpenes in heterologous expression systems.

Place, publisher, year, edition, pages
2016. Vol. 38, 159-169 p.
Keyword [en]
ABC transporters, Artemisia annua, Artemisinin, Lipid transfer proteins, Nicotiana benthamiana, Pleiotropic Drug Resistance protein
National Category
Biochemistry and Molecular Biology
Research subject
Chemistry, Biochemistry
Identifiers
URN: urn:nbn:se:lnu:diva-56057DOI: 10.1016/j.ymben.2016.07.004ISI: 000387984600017PubMedID: 27421621Scopus ID: 2-s2.0-84982854633OAI: oai:DiVA.org:lnu-56057DiVA: diva2:971492
Available from: 2016-09-16 Created: 2016-08-31 Last updated: 2016-12-14Bibliographically approved

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Olofsson, LindaBrodelius, Peter E.
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CiteExportLink to record
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Citation style
  • apa
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More styles
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