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  • 1.
    Carlsson, Stina K.
    et al.
    Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
    Edman, Maria
    Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
    Delbro, Dick
    Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
    Gierow, Peter
    Linnaeus University, Faculty of Science and Engineering, School of Natural Sciences.
    Adenosine A2 receptor presence, and synergy with cholinergic stimulation in rabbit lacrimal gland2010In: Current Eye Research, ISSN 0271-3683, E-ISSN 1460-2202, Vol. 35, no 6, p. 466-474Article in journal (Refereed)
    Abstract [en]

    PURPOSE: Secretion from the lacrimal gland is an important part of the well-being of the eye, and a central part in the search for treatment of dry eye syndrome. Adenosine has stimulatory effects on the lacrimal gland, and can potentiate the effect of the cholinergic agonist carbachol (Cch). The aim of the present study is to investigate the presence of the adenosine A(2) receptor subtypes A(2A) and A(2B) in the rabbit lacrimal gland, and to characterize their role in regulated acinar cell secretion.

    METHODS: Expression of the receptors was investigated using reverse transcriptase-PCR (RT-PCR) and immunofluorescence, and secretion effects were studied using a secretion assay in isolated lacrimal gland acinar cells.

    RESULTS: Presence of both receptors was detected by RT-PCR and immunofluorescence. The secretion assay revealed a minor effect of stimulation of the A(2) receptors, and a strong synergistic effect with the cholinergic agonist Cch. The synergistic effect was significantly reduced by the A(2B) antagonist PSB 1115, but not by the A(2A) antagonist SCH 58261, indicating that A(2B) is the receptor responsible for this potentiation.

    CONCLUSIONS: The study reveals the presence of the adenosine A(2) receptor subtypes as well as a role for them in lacrimal gland secretion, and especially in the synergy with purinergic and cholinergic stimulation.

  • 2.
    Delbro, Dick
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Heiman, J
    Wallin, M
    Gustafsson, BI
    Friman, S
    Pharmacological preconditioning of rat liver by up-regulation of heme oxygenase 1.2006In: Transplant Proc 38, 2006Conference paper (Refereed)
  • 3.
    Delbro, Dick
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nylund, Gunnar
    Nordgren, Svante
    Demonstration of P2Y4-purinoceptors in the HT-29 human colon cancer cell line.2005In: Auton Autacoid Pharmacol, Vol. 25, p. 163-166Article in journal (Refereed)
  • 4.
    Edman, Maria
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Andersson, Sofia
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Gierow, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Functional expression of the adenosine A1 receptor in rabbit lacrimal gland.2008In: Experimental Eye Research, ISSN 0014-4835, E-ISSN 1096-0007, Vol. 86, no 1, p. 110-117Article in journal (Refereed)
  • 5. Giglio, D
    et al.
    Andersson, M
    Aronsson, P
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Haraldsson, B
    Tobin, G
    Changes in muscarinic receptors in the toad urothelial cell line TBM-54 following acrolein treatment.2008In: Clinical and experimental pharmacology & physiology, ISSN 0305-1870, E-ISSN 1440-1681, Vol. 35, p. 217-222Article in journal (Refereed)
  • 6. Giglio, D
    et al.
    Ryberg, A T
    To, K
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Tobin, G
    Altered muscarinic receptor subtype expression and functional responses in cyclophosphamide induced cystitis in rats2005In: Autonomic neuroscience : basic & clinical, Vol. 122 (1-2), p. 9-20Article in journal (Refereed)
  • 7. Khorram-Manesh, A
    et al.
    Ardakani, J V
    Behjati, H R
    Nylund, G
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    The effect of opioids on the development of postoperative intra-abdominal adhesions2006In: Digestive diseases and sciences, Vol. 51, no 3, p. 560-565Article in journal (Refereed)
  • 8. Nylund, Gunnar
    et al.
    Hultman, Lena
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    P2Y2- and P2Y4 purinergic receptors are over-expressed in human colon cancer.2006In: Auton Autacoid Pharmacol, Vol. 27, p. 79-84Article in journal (Refereed)
  • 9. Nylund, Gunnar
    et al.
    Nordgren, Svante
    Andersson, Marianne
    Haraldsson, Börje
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Effects of noradrenaline or prostaglandin E2 on on-line measurement of extracellular acidification rate of MCG 101, or K1735-M2 tumour cells.2004In: Life Sci, p. 1747-1759Article in journal (Refereed)
  • 10. Nylund, Gunnar
    et al.
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Demonstration of functional receptors for noradrenaline and adenosine-5´-triphosphate, but not for prostaglandin E2, in HT-29 human colon cancer cell line.2003In: Auton Autacoid Pharmacol, Vol. 23, p. 193-199Article in journal (Refereed)
  • 11. Nylund, Gunnar
    et al.
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Expression of P2Y(2) purinoceptors in MCG 101 murine sarcoma cells, and HT-29 human colon carcinoma cells.2004In: Autonomic Neurosci, Vol. 112, p. 69-79Article in journal (Refereed)
  • 12. Nylund, Gunnar
    et al.
    Pettersson, Ann
    University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden.
    Bengtsson, Cecilia
    Khorram-Manesh, Amir
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, Göteborg, SE-413 45, Sweden.
    Functional expression of µ-opioid receptors in the human colon cancer cell line, HT-29, and their localization in human colon.2008In: Digestive Diseases and Sciences, ISSN 0163-2116, E-ISSN 1573-2568, Vol. 53, no 2, p. 461-466Article in journal (Refereed)
    Abstract [en]

    We have investigated the functional expression of μ-opioid receptors (MORs) in the human colon cancer cell line, HT-29. As revealed by immunocytochemistry, immunoreactivity was present in both the cytoplasm and nuclei of the cells. Challenge with morphine for 24 h (1 nM to 1 μM) barely affected cell proliferation, while the secretion of urokinase type plasminogen activator (a protease involved in invasion/metastasis) was markedly augmented by a concentration of 0.1 μM. Human colon cancer tissue from 14 consecutively operated patients was investigated by immunohistochemistry. MORs were found in the nuclei of colonocytes and immune cells of the lamina propria in tumor-free tissue. In tumor tissue, immunoreactivity was found in the membrane and often in the nuclei of tumor cells. The current findings suggest that morphine administration could affect tumor progression by interfering with, for example, invasive properties. Our demonstration of a nuclear expression of the MORs appears to be a novel finding.

  • 13.
    Pettersson, Ann
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nilsson, Linn
    Nylund, Gunnar
    Khorram-Manesh, Amir
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden.
    Is acetylcholine an autocrine/paracrine growth factor via the nicotinic α7-receptor subtype in the human colon cancer cell line HT-29?2009In: European Journal of Pharmacology, ISSN 0014-2999, E-ISSN 1879-0712, Vol. 609, no 1-3, p. 27-33Article in journal (Refereed)
    Abstract [en]

    We used immunochemistry to demonstrate expression of acetylcholine's nicotinic α7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine–or speculatively, even intracrine–signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.

  • 14.
    Pettersson, Ann
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nordlander, S
    Nylund, G
    Khorram-Manesh, A
    Nordgren, S
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, SE-413 45 Gothenburg.
    Expression of the endogenous, nicotinic acetylcholine receptor ligand, SLURP-1, in human colon cancer.2008In: Autonomic & Autacoid Pharmacology, ISSN 1474-8665, E-ISSN 1474-8673, Vol. 28, no 4, p. 109-116Article in journal (Refereed)
    Abstract [en]

    1. Secreted mammalian Ly-6/urokinase plasminogen activator receptor-related protein-1 (SLURP-1) is a recently discovered endogenous ligand at the alpha7 subunit of the nicotinic acetylcholine receptors. Previous reports have shown that SLURP-1 is expressed in normal human keratinocytes seemingly with a pro-apoptotic function. Conversely, such expression was markedly attenuated in transformed cells and it was suggested that the molecule could convey protection against malignant transformation. 2. In this study, we demonstrated the mRNA expression (by RT-PCR) and protein expression (by Western blotting and immunocytochemistry) of SLURP-1 in the human colon cancer cell line, HT-29. 3. Furthermore, we demonstrated the expression of SLURP-1 (by immunohistochemistry) in tumour cells of human colon cancer tissue, and, to a greater extent, in immune and smooth muscle cells of adjacent, macroscopically tumour-free colon tissue. 4. The current findings suggest that SLURP-1 participates in the regulation of gut immune functions and motility, as well as possibly playing a role in colon carcinogenesis/cancer progression.

  • 15.
    Pettersson, Ann
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nylund, Gunnar
    Khorram-Manesh, Amir
    Nordgren, Svante
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences. Department of Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Nicotine induced modulation of SLURP-1 expression in human colon cancer cells.2009In: Autonomic Neuroscience: Basic & Clinical, ISSN 1566-0702, E-ISSN 1872-7484, Vol. 148, no 1-2, p. 97-100Article in journal (Refereed)
    Abstract [en]

    The secreted mammalian Ly-6⁄urokinase plasminogen activator receptor-related protein-1 (SLURP-1) is an endogenous ligand at the α7 subunit of the nicotinic acetylcholine receptor (nAChR). SLURP-1 has anti-tumourigenic properties. In the current study, we demonstrate that the challenge of HT-29 human colon cancer cells with nicotine for 24 h to increase cell growth via the α7nAChRs, caused a marked reduction of the protein expression of SLURP-1. We suggest that there is an interplay between acetylcholine and SLURP-1 in the HT-29 cells, both molecules serving as autocrine growth controlling ligands at the α7nAChR, where acetylcholine regulates the release of SLURP-1.

  • 16. Wang, Wen-hua
    et al.
    Svanberg, Elisabeth
    Delbro, Dick
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lundholm, Kent
    NOS isoenzyme content in brain nuclei as related to food intake in experimental cancer cachexia.2005In: Brain Res, Vol. 134, p. 205-214Article in journal (Refereed)
1 - 16 of 16
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