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  • 1.
    Ölje, Elin
    Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences.
    Metodjämförelse mellan DiffMaster Octavia och CellaVision DM1200 avseende differentialräkning av leukocyter: en viktig analys inom vården2016Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Leukocytes, white blood cells, are cells of the immune system. They are produced and derived from hematopoietic stem cells in the bone marrow. Leukocytes can be divided into neutrophils, lymphocytes, monocytes, eosinophils and basophils. To determine the numbers of leukocytes in blood the leukocyte particle concentration (B-LPK) can be analyzed by cell counters. When B-LPK is elevated or lowered a differential count of leukocytes (B-Diff) is performed to see in which cell systems the change exists. The manual analysis involves peripheral blood smears stained with a cytochemical color, May-Grünwald Giemsa. The smear examined in an automatic microscopically system that counts, photographs and pre-classify leukocytes by its appearance. DiffMaster Octavia and CellaVision DM1200 are two variants of such instruments from the same manufacturer (CellaVision AB, Lund, Sweden). The aim of the study was to do a comparison between these instruments by analyzing 60 samples consisting venous blood in EDTA-tubes. The samples were collected randomly from patients (32 men and 28 women) between 19-95 years old. The results from two-sided paired t-test showed no significant difference between the differential count of neutrophils, lymphocytes and monocytes. The correlation was 0,95, 0,91 and 0,68. However, there was a significant difference between the instruments differential count of eosinophils and basophils, the correlation was 0,91 and 0,20. When counting only 200 cells a profit of 2 % distinguish up to 1-5 %. Abnormalities in leukocytes which represents only a few percent in blood can therefore be very large. Method comparison showed that both instruments give the same results and are considered equivalent in analysis of manual B-Diff.

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