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  • 301. Björk, Sven
    et al.
    Ekstam, Börje
    University of Kalmar, School of Pure and Applied Natural Sciences.
    The Stentsovsko-Zhebriansky Plavni restoration plan1996Report (Other academic)
  • 302. Björkman, J
    et al.
    Ellström, Patrik
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Andersson, D I
    Hughes, D
    Novel ribosomal mutations affecting translational accuracy, antibiotic resistance and virulence of Salmonella typhimurium1999In: Molecular microbiology, Vol. 31, p. 53-58Article in journal (Refereed)
  • 303. Blackburn, N.
    et al.
    Azam, F.
    Hagström, Åke
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Spatially explicit simulations of a microbial food web.1997In: Limnology and Oceanography, ISSN 0024-3590, E-ISSN 1939-5590, Vol. 42, p. 613-622Article in journal (Refereed)
  • 304. Blackburn, N.
    et al.
    Wikner, J.
    Cuadros Hanson, R.
    Bjørnsen, K.P.
    Hagström, Åke
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Rapid determination of bacterial abundance, biovolume, morpology and growth by neural network based image analysis.1998In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 64, p. 3246-3255Article in journal (Refereed)
  • 305. Blackburn, N.
    et al.
    Zweifel, Ulla Li
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Hagström, Åke
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Cycling of marine dissolved organic matter: II. A model analysis.1996In: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 11, p. 79-90Article in journal (Refereed)
  • 306.
    Blinkowska, Nathalie
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Kan man påvisa något samband mellan högt sojaintag och bröstcancerrisk?2009Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
  • 307.
    Blom, Jonas
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lilja, Clas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    A comparative study of embryonic development of some species some birds with diffrent patterns of postnatal growth2005In: Zoology, Vol. 108 (2), p. 81-95Article in journal (Refereed)
  • 308.
    Blom, Jonas
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lilja, Clas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    A comparative study of growth, skeletal development and eggshell composition in some species of birds2004In: Journal of Zoology London. Oxford University Press, Vol. 262Article in journal (Refereed)
  • 309.
    Blom, Jonas
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lilja, Clas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Heterochrony and the developmental origins of neonatal characteristics of precocial and altircial birds.2006In: Journal of Ornithology 147 (5), 2006Conference paper (Refereed)
  • 310.
    Blom, Jonas
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lilja, Clas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Patterning of embryonic development in birds2004In: Journal of Morphology 260, 2004Conference paper (Refereed)
  • 311.
    Blom, Jonas
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Österström, Ola
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lilja, Clas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Embryonic stages in the development of the altricial Fieldfare2006In: Journal of Orhithology. 147 (5). 139, 2006Conference paper (Refereed)
  • 312.
    Blomberg, Carolina
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Intrathecal and Systemic Complement Activation Studies of Multiple Sclerosis and Guillan-Barré Syndrome2009Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    Both Multiple Sclerosis (MS) and Guillan-Barré syndrome (GBS) are neurological inflammatory demyelinating autoimmune diseases, with a probable antibody contribution. Complement proteins in both MS and GBS does play a role in inflammation and demyelination at pathogenesis, according to earlier scientific evidence. The aim of this examination project work was to investigate systemic and intrathecal complement activation in MS and GBS, to gain further knowledge that might be useful for development of future therapeutics targeting immune responses during those diseases. An additional aim was to develop a new ELISA method for detection of complement iC3.

    By using sandwich ELISA, complement proteins C1q, C4, C3, fH and C3a were measured in plasma and cerebrospinal fluid (CSF) from persons within 4 different diagnostic groups; MS, other neurological diseases (OND), GBS and controls (C). An ELISA method to detect iC3 (hydrolysed C3) was also developed, including usage of SDS-PAGE. Results based on raw data and statistical analysis show significantly elevated levels of C3a (C3a/C3) in MS and decreased C3 in plasma. In CSF low levels of C4 and C3a/C3 in MS were detected, though correlation of C3a and C1q was positive. GBS reveal high levels of all complement proteins analysed in CSF except for C3, and a positive correlation of C3a and C1q as well as C3a and fH was found.

    These results indicate that MS patients have systemic complement activation; however the activation pathway is not determined. Complement activation in MS may also occur intrathecally, with correlation analysis indicating a possible activation via the classical pathway. MS patients suffering from a more acute relapsing-remitting (RR) MS have a more prominent systemic complement activation compared to MS patients responding to beta-interferon treatment. Systemic increased C3a/C3 ratio may be a possible biomarker to distinguish more acute RR MS in an earlier step of MS pathogenesis and should be further investigated. GBS patients have an intrathecal complement activation that seems to occur via the classical pathway.

  • 313.
    Blomster, Linda
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Role of fractalkine/CX3CL1 signalling in the regenerating olfactory epithelium2008Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The olfactory epithelium (OE) is a useful model to study neurogenesis because of the sustained ability for self-repair throughout adult life. The main aim of this study was to investigate putative neuroprotective roles of fractalkine/CX3CL1 signalling in the olfactory epithelium after experimentally-induced cell death and replacement of olfactory sensory neurons. Previous studies have shown that signalling through the fractalkine receptor, CX3CR1, can regulate neurotoxicity of monocyte-derived cells via suppression of pro-inflammatory cytokines production, i.e. IL-1β, TNF-α and IL-6. This is particularly interesting as the latter molecules contribute to a microenvironment that causes neuronal death and impaired neurogenesis.

    Real-time PCR (qPCR) was used to investigate differential expression of pro-inflammatory cytokines in wild-type and CX3CR1-deficient mice following olfactory bulbectomy. In addition, immunohistochemistry was used to investigate the influx of phagocytic macrophages into the OE and the extent of neurogenesis following injury. Increased numbers of intraepithelial macrophages were detected in the olfactory epithelium of CX3CR1-deficient mice after injury. Interestingly, expression levels of OMP (a marker for mature olfactory sensory neurons) were significantly reduced in CX3CR1-deficient mice after injury, which is indicative for increased neuronal death. The latter was confirmed by quantitative counts of OMP-positive neurons in tissue sections. The increased expression levels of both TNF-α and IL-6 that were detected in CX3CR1-deficient mice likely contributed to this aggravated neuronal death. The extent of neurogenesis was significantly decreased in the CX3CR1-deficient mice compared to the wild-type mice after bulbectomy.

    In summary, these results suggest that fractalkine signalling in the olfactory epithelium may have an important role in the regulation of macrophage responses to injury and maintenance of an environment that allows for functional repair.

  • 314.
    Blüthl-Larsson, Dinah
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Emmetropisering ochrefraktionsutveckling hos barn samtförväntade värden vid 9-11 års ålder2009Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    Syfte: Syftet med denna studie var att ta reda på vad man kan förvänta sig för refraktionsfel av barn genom att undersöka en åldersgrupp från nio till elva år med Mohindra närretinoskopi. Resultaten skulle sedan jämföras med fem andra studier gjorda inom området. Syftet var vidare att fördjupa sig inom emmetropisering och refraktionsutveckling.

    Metod: 77 barn från tre skolor i Vadstena kommun i åldrarna 9 till 11 år undersöktes under april 2009 med Mohindra närretinoskopi. De sfäriska ekvivalenta refraktionsmedelvärdena togs fram och jämfördes med likvärdiga resultat från fem andra studier genomförda i olika länder.

    Resultat: De sfäriska ekvivalenta medelvärdena var för 9-, 10- och 11-åringar +1.29 D, +0.97 D respektive +1.22 D för pojkarna och +1.00 D, +1.22 D respektive +0.92 D för flickorna i Vadstena. I de asiatiska studierna var medelvärdena mot mer myopi medan den svenska och chilenska studien liknade medelvärdena från studien i Vadstena.

    Slutsats: Resultatet av denna undersökning visar att refraktionsutvecklingen tenderar att gå mot mer negativa värden med ökande ålder, dock är det långt ifrån alla barn som blir myopa när de börjar skolan. Asiatiska barn visar myopa medelvärden av refraktionen medan bland annat svenska barn visar hyperopa värden.

  • 315.
    Bodin, Per
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Wiman, Bo
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Resilience and other stability concepts in ecology: notes on their origin, validity and usefulness2004In: The ESS Bulletin, Vol. 2 (2), p. 33-43Article in journal (Refereed)
  • 316.
    Bodin, Per
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Wiman, Bo
    University of Kalmar, School of Pure and Applied Natural Sciences.
    The usefulness of stability concepts in forest management when coping with increasing climate uncertainties2007In: Forest Ecology and Management, ISSN 0378-1127, E-ISSN 1872-7042, Vol. 242, p. 541-552Article in journal (Refereed)
  • 317. Bohman, B
    et al.
    Nordlander, G
    Nordenhem, H
    Sunnerheim, K
    Borg-Karlson, A-K
    Unelius, Rikard
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Structure - activity relationships of phenyl propanoids as antifeedants for the pine weevil Hylobius abieist2008In: Journal of Chemical Ecology, ISSN 0098-0331, E-ISSN 1573-1561, Vol. 34, no 3, p. 339-352Article in journal (Refereed)
  • 318.
    Bohman, Björn
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Cavonius, Lillie
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Unelius, C. Rikard
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Vegetables as biocatalysts in stereoselective hydrolysis of labile organic compounds2009In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270, Vol. 11, no 11, p. 1900-1905Article in journal (Refereed)
    Abstract [en]

    Hydrolysis of labile esters of beta-hydroxyketones has been performed with whole plant tissue from various vegetables. The pheromone 5-hydroxy-4-methyl-3-heptanone (1) was used as the model compound. Hydrolysis of acetates and benzoates of 1 was unsuccessful using normal conditions of ester hydrolysis, both by chemical hydrolysis and by the means of commercial lipases. When, however, whole cells of carrot, celery root, eggplant, parsley root, parsnip and potato were used as reagents, hydrolysis of the acetates was successful. At low conversion the hydrolysis was stereoselective and at total conversion virtually no formation of by-products was observed. The selectivity varied among the eight vegetables that were evaluated. Methods of preparation and substrate-to-plant ratio were examined. Furthermore, acetates and benzoates of three analogous compounds [5-hydroxy-3-heptanone (2), 5-hydroxy-5-methyl-3-heptanone (3) and 5-ethyl-6-hydroxy-4-octanone (4)] were hydrolyzed by potato and sweet potato to various degrees, indicating that the method is general for the mild and stereoselective hydrolysis of secondary beta-alkoxy-and beta-aryloxyketones.

  • 319.
    Bohman, Björn
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Unelius, C. Rikard
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Synthesis of all four stereoisomers of 5-hydroxy-4-methyl-3-heptanone using plants and oyster mushrooms2009In: Tetrahedron, ISSN 0040-4020, E-ISSN 1464-5416, Vol. 65, no 42, p. 8697-8701Article in journal (Refereed)
    Abstract [en]

    All four possible stereoisomers of 5-hydroxy-4-methyl-3-heptanone were synthesized from common achiral reagents using fast, straightforward organic synthesis, including the use of whole tissue of Daucus carota, Solanum melongena, and Pleurotus ostreatus.

  • 320.
    Bohman, Irene
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Coarse detritus in oligotrophic lake littoral zones: utilization by intervertebrates and contribution to carbon flow2005Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    The general aim of this thesis was to increase the understanding of the mechanisms behind coarse detritus turnover in oligotrophic lake littoral zones. The reason for this interest is that small lakes located in forested areas often display dependence on detritus based energy sources, both external and internal. Additionally, in lake ecosystems the coarse detritus resource has been comparatively less studied than in streams. Both dissolved and particulate external detritus constitute extra energy contribution to ecosystems, but only if some organisms can assimilate the provided matter. The most common capable organisms to do so in freshwaters are bacteria, fungi and invertebrates, and they return a variety of intermediary products available for other consumers. Further, it has been suggested that a positive relation between species diversity and ecosystem function exists. Therefore the study of both community and individual species of decomposers may provide information significant to understand changes in ecosystem function.

    Within the outline of the thesis, I studied what species are involved in the processing of coarse detritus carbon and what are the key routes for the further use of this littoral carbon. I specially emphasize the temporal pattern of the detritus turnover and the availability of decomposition products to other organisms in the lake ecosystem.

    The results showed that microorganisms and invertebrates in the lake littoral zone successively process carbon from coarse detritus during the whole year. The weight loss from coarse detritus displayed a seasonal distinctive pattern temporally connected to shredder appearance and growth. Therefore, I emphasize the key role of certain trichopteran shredder species for litter processing in lakes in this region, southeast Sweden. At the same time, the shredder function of Asellus aquaticus was less important than expected. A. aquaticus was shown to utilize of a quite broad range of food sources and to prefer other habitats during the main decomposition period for leaf litter. In the laboratory I discovered that the decomposition of leaf litter principally results in two products, DOM from passive leakage and FPOM from active leaf litter processing by shredders. The experiment showed that shredders have the potential to consume leaf litter resources before microbial decomposition influence litter weight considerably. Thus, the quantitative importance of microorganisms (fungi and/or bacteria) for leaf litter turnover is suggested to directly depend on density of shredders in lake littoral zones of the kind studied. I have also shown that the combination of detritus types and invertebrate processor species influence the temporal accessibility of intermediate decomposition products.

    I conclude that coarse detritus is an attractive food resource for invertebrates also in small oligotrophiclakes like it is in smaller streams. I also conclude, that the lake specific set of shredder species recycle this carbon resource yearly in a fine-scaled temporal sequence - adapted to the predictable seasonal variation of detritus quality on these latitudes. Further, I stress the importance of increased knowledge of decomposing processes before predicting the effects of disturbance on this kind of widespread freshwater systems. Finally, I propose that the decomposition rate of coarse detritus should be tested as an assessment tool for integrating disturbances on ecosystem functions in small oligotrophic lakes, respecting the unique lake characteristics.

  • 321.
    Bohman, Irene
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Comparison of temporal weight loss patterns between two decomposing aquatic macrophytesManuscript (Other (popular scientific, debate etc.))
  • 322.
    Bohman, Irene
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Bjelke, Ulf
    Seasonal variation of food sources in lake littoral benthic fauna, as indicated by stable isotopesManuscript (preprint) (Other academic)
  • 323.
    Bohman, Irene
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Herrmann, Jan
    University of Kalmar, School of Pure and Applied Natural Sciences.
    The timing for winter-growing shredder species and leaf litter turnover rate in an oligotrophic lake, SE Sweden2006In: Hydrobiologia, ISSN 0018-8158, E-ISSN 1573-5117, Vol. 556, no 1, p. 99-108Article in journal (Refereed)
    Abstract [en]

    Small freshwater systems often depend on allochthonous organic subsidies to sustain productivity. Benthic invertebrates consuming coarse detritus maintain the energy flow by conveying dead organic matter into prey items and increase the food availability for other consumers. Compared to lotic systems, the dynamics of coarse detritus decomposition has not received much attention in lakes. The objectives of this study were to investigate the seasonality of leaf litter turnover and the timing of abundance of potential shredder species in a typical oligotrophic boreal lake. Leaf litter was experimentally exposed in litterbags in the littoral zone in Lake Välen from autumn to late spring two consecutive years. The weight loss rate of leaf litter initially followed the same pattern during both winter periods, but was markedly influenced by freezing in late winter the second year. Further, the seasonal variation patterns in abundance in litterbags were quite different among the potential shredder species. Only the limnephilid caddis larvae showed a density variation pattern possible to connect to the weight loss of leaf litter in litterbags. Otherwise frequent detritivores such as Asellus aquaticus and Leptophlebia marginata displayed lowest density in litterbags during the main weight loss period. However, after the long ice period the second winter the remaining leaf litter seemed to be consumed by A. aquaticus. With increasing knowledge of the initial leaf breakdown process and the guild of shredders in lakes, the decomposition rate may also in this habitat become a useful instrument when evaluating the impact from perturbations on ecosystem function.

  • 324.
    Bohman, Irene
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Tranvik, Lars
    The effects of shredding invertebrates on the transfer of organic carbon from littoral leaf litter to water-column bacteria2001In: Aquatic Ecology, ISSN 1386-2588, Vol. 35, no 1, p. 43-50Article in journal (Refereed)
  • 325. Boman, Anton
    et al.
    Åström, Mats
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Fröjdö, Sören
    Sulfur dynamics in boreal acid sulphate soils rich in metastable iron sulfide – The role of artificial drainage2008In: Chemical Geology, ISSN 0009-2541, E-ISSN 1872-6836, Vol. 255, no 1-2, p. 68-77Article in journal (Refereed)
    Abstract [en]

    The sulfur dynamics of boreal brackish-water sediments rich in metastable iron sulfide (average elemental composition of FeS1.1), which upon oxidation have a huge impact on stream-water chemistry, were studied. Two cores, extending from the upper oxidized layer (acid sulfate soil) into the underlying iron sulfide-bearing sediment (potential acid sulfate soil) were collected at a site close to the sea level and at another site about 40 m above the latter in a region of current isostatic land uplift (Finland). The data clearly show, in contrast to what is often argued, that these notorious acidic soils are formed not as a result of the natural uplift but because of extensive ditching of farmlands. Above the depth of artificial drainage, S and Ni are abundantly lost, while beneath that level pyrite is abundant and metastable iron sulfide is increasing with depth, and the Ni concentration is relatively stable. In the narrow zone between the reduced and oxidized layers, the processes are dynamic and result in preservation of elemental S at one of the locations. The sulfur isotopic composition for metastable iron sulfide and pyrite in the investigated acid sulfate soils were found to be distinctly bimodal and roughly corresponding to a similar distribution in stream-water sulfate earlier reported from the same region. This indicates that pyrite is the main source of sulfate and acidity in some affected drains, while in others it is metastable iron sulfide.

  • 326. Bondestam, K.
    et al.
    Vasari, A.
    Vasari, Y.
    Lemdahl, Geoffrey
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Eskonen, K.
    Younger Dryas and Preboreal in Salpausselkä foreland, Finnish Karelia.1994In: Dissertationes Botanicae, ISSN 0070-6728, Vol. 234, p. 161-206Article in journal (Refereed)
  • 327. Bonnedahl, Jonas
    et al.
    Broman, Tina
    Waldenström, Jonas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Palmgren, Helena
    Niskanen, Taina
    Olsen, Björn
    University of Kalmar, School of Pure and Applied Natural Sciences.
    In search of human-associated bacterial pathogens in Antarctic wildlife: report from six penguin colonies regularly visited by tourists2005In: Ambio, Vol. 34, p. 424-426Article in journal (Refereed)
  • 328.
    Bonnedahl, Jonas
    et al.
    Uppsala University ; Kalmar County Hospital.
    Drobni, M
    Gauthier-Clerc, M
    Hernandez, Jorge
    Kalmar County Hospital.
    Granholm, S
    Kayser, Y
    Melhus, Å
    Kahlmeter, G
    Waldenström, Jonas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Johansson, A
    Olsen, Björn
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Dissemination of Escherichia coli with CTX-M Type ESBL between Humans and Yellow-Legged Gulls in the South of France2009In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 4, no Article number: e5958Article in journal (Refereed)
    Abstract [en]

     Extended Spectrum beta-Lactamase (ESBL) producing Enterobacteriaceae started to appear in the 1980s, and have since emerged as some of the most significant hospital-acquired infections with Escherichia coli and Klebsiella being main players. More than 100 different ESBL types have been described, the most widespread being the CTX-M beta-lactamase enzymes (bla(CTX-M) genes). This study focuses on the zoonotic dissemination of ESBL bacteria, mainly CTX-M type, in the southern coastal region of France. We found that the level of general antibiotic resistance in single randomly selected E. coli isolates from wild Yellow-legged Gulls in France was high. Nearly half the isolates (47,1%) carried resistance to one or more antibiotics (in a panel of six antibiotics), and resistance to tetracycline, ampicillin and streptomycin was most widespread. In an ESBL selective screen, 9,4% of the gulls carried ESBL producing bacteria and notably, 6% of the gulls carried bacteria harboring CTX-M-1 group of ESBL enzymes, a recently introduced and yet the most common clinical CTX-M group in France. Multi locus sequence type and phylogenetic group designations were established for the ESBL isolates, revealing that birds and humans share E. coli populations. Several ESBL producing E. coli isolated from birds were identical to or clustered with isolates with human origin. Hence, wild birds pick up E. coli of human origin, and with human resistance traits, and may accordingly also act as an environmental reservoir and melting pot of bacterial resistance with a potential to re-infect human populations.

  • 329. Bonnedahl, Jonas
    et al.
    Olsen, Björn
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Waldenström, Jonas
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Broman, Tina
    Jalava, J
    Huovinen, P
    Österblad, Monika
    Antibiotic susceptibility of faecal bacteria in Antarctic penguins2008In: Polar Biology, ISSN 0722-4060, E-ISSN 1432-2056, Vol. 31, p. 759-763Article in journal (Refereed)
  • 330.
    Borg, Caroline
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Variationen i corneal kurvatur över dagen2009Independent thesis Basic level (degree of Bachelor)Student thesis
    Abstract [sv]

    För att en skarp bild ska kunna avbildas på retina krävs det att cornea och lins är transparenta men även att de innehar kvalitativa egenskaper i brytning. Fel i corneal kurvatur är en av orsakerna till refraktiva fel hos ögat. Kurvaturen kan mätas med en keratometer eller en topograf, vilken den sist nämnda även har stor betydelse bland annat vid kontaktlinstillpassning, refraktiv kirurgi och till hjälp vid diagnostisering av flera sjukdomstillstånd.

    Syfte: Syftet med den här studien var att undersöka om kurvaturen ändras under dagen, på en 8-timmars period, och i så fall hur mycket.

    Metod: Corneal kurvatur studerades på 16 ögon, 7 män och 9 kvinnor, med en median ålder på 24 år. Mätningarna utfördes med en Topcon CA-100F Corneal Analyzer, tre gånger under en dag, med fyra timmars mellanrum. Nio punkter på cornea jämfördes specifikt, vilka var apex samt 1 och 2 mm därifrån; superiort, temporalt, inferiort och nasalt.

    Resultat: Studien visade att cornea var flatast på morgonen och blev kupigare under dagen på alla mätpunkter förutom superiort. Förändringen var större på punkterna 2 mm från apex än 1 mm i alla riktningar förutom den temporala. Den totala variationen i snitt för alla punkter var en kurvaturminskning på 0,0332 ±0,07 mm och en genomsnittlig ökning i brytkraft på 0,17 ±0,35 D.

    Slutsats: Studien visar en signifikant förändring i corneal kurvatur och brytkraft under dagen. Dock var variationen i kurvatur individuell och därför ses en viktig roll i att ta topografibilder på varje enskild patient.

  • 331. Borg-Karlson, A K
    et al.
    Nordlander, R
    Mudalige, A
    Nordenhem, Henrik
    Unelius, Rikard
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Antifeedants in the feces of the pine weevil Hylobius abietis: Identification and biological activity2006In: Journal of chemical ecology, Vol. 32, no 5, p. 943-957Article in journal (Refereed)
  • 332.
    Boström, Kjärstin H.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nitrogen fixation among marine bacterioplankton2006Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    While bacterioplankton indisputably control vital biogeochemical paths in the cycling of carbon and nutrients in the world’s oceans, our knowledge about the functional and genetic diversity of bacterioplankton communities is negligible. In this thesis, molecular and more traditional microbiological methods were used to study the specific function of N2-fixation and in a general sense diversity of marine bacterioplankton species.

    Most oceans are nitrogen limited and, therefore, adaptive to bacterioplankton capable of N2-fixation. Recent studies have found nifH genes (coding for the nitrogenase enzyme) related to diverse heterotrophic bacteria in oceanic seawater samples indicating that, along with cyanobacteria, also heterotrophic bacteria benefit from N2-fixation. Here, molecular and cultivation methods were used to examine diazotrophic bacterioplankton in the Baltic Sea. We successfully isolated heterotrophic N2-fixing bacteria belonging to the γ-proteobacterial class by means of low-nitrogen plates and semi-solid diazotrophic medium tubes. The isolates required low-O2 conditions for N2-fixation. Using Real-time PCR it was found that heterotrophic bacterioplankton carrying the nifH gene was abundant (3 x 104 nifH gene copies L seawater-1) at locations in the Southwest Baltic proper.

    With the aim to identify the main N2-fixing organisms in Baltic Proper surface waters, a clone library of nifH gene transcripts (RNA) was generated. Clone inserts were exclusively related to Aphanizomenon sp. and Nodularia sp. Using quantitative real-time PCR it was found that the nifH gene expression from Nodularia sp. was highly variable between stations in the Baltic Proper but was 10-fold higher during mid summer relative to early summer and fall. A diel study showed a 4-fold increase in Nodularia transcript concentrations at early to mid day relative to rest of the day. Real-time PCR was found to be a powerful and highly sensitive method for measuring gene expression.

    Since nucleic acids are a prerequisite for molecular analyses of bacterioplankton dynamics a protocol to extract DNA from seawater samples was developed with the aim to maximize the yield of high-quality DNA. Each step in the protocol was important for the efficiency of extraction. The obtained extraction efficiencies were up to 92% for seawater samples and up to 96% for isolates. The protocol provides a guideline for DNA extraction from seawater samples for other studies.

    In a global sampling campaign (9 locations from polar, tropical and temperate regions) we sampled DNA from surface water and constructed 16S rRNA gene libraries to investigate diversity and biogeography of bacterioplankton. Approx. 80% of the sequences found were similar to sequences already deposited in GenBank, indicating that a large fraction of the marine bacterioplankton already has been sampled, which in turn suggests a limited global bacterioplankton diversity.

    This thesis have improved our knowledge about the composition and nifH gene expression of the diazotrophic bacterioplankton community in the Baltic Sea and contribute significantly to the discussion on global marine bacterioplankton diversity and biogeography.

  • 333.
    Boström, Kjärstin H.
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Reimann, Lasse
    Kühl, Michael
    Hagström, Åke
    Isolation and gene quantification of heterotrophic N2-fixing bacterioplankton in the Baltic Sea2007In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 9, no 1, p. 152-164Article in journal (Refereed)
  • 334.
    Boström, Kjärstin H.
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Riemann, Lasse
    Zweifel, Ulla Li
    Hagström, Åke
    Nodularia sp. nifH gene transcripts in the Baltic Sea proper2007In: Journal of Plankton Research, ISSN 0142-7873, E-ISSN 1464-3774, Vol. 29, no 4, p. 391-399Article in journal (Refereed)
  • 335.
    Boström, Kjärstin
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Simu, Karin
    Hagström, Åke
    Riemann, Lasse
    Optimization of DNA extraction for quantitative marine bacterioplankton community analysis2004In: Limnology and oceanography: methods, Vol. 2, p. 365-373Article in journal (Refereed)
  • 336. Bramble, J L
    et al.
    Graves, D J
    Brodelius, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Plant Cell Culture using a Novel Bioreactor: The Magnetically Stabilized Fluidized Bed1990In: Biotechnology progress (Print), ISSN 8756-7938, E-ISSN 1520-6033, Vol. 6, no 6, p. 452-457Article in journal (Refereed)
    Abstract [en]

    A novel bioreactor using magnetically stabilized fluidized bed (MSFB) technology has been developed that has certain advantages for cultivating cells continuously. In this system, the cells are protected from shear and are constrained to move through the fermenter in lock-step fashion by being immobilized in calcium alginate beads. The MSFB permits good mass transfer, minimizes particle collisions, and allows for the production of cells while maintaining a controlled cell residence time. Details of the experimental system are described. In addition, the experimental performance of an MSFB used to grow plant cells in batch mode is compared to the results obtained in shake flask culture. 

  • 337. Brand, S J
    et al.
    Morise, Z
    Tågerud, Sven
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Mazzola, L
    Granger, D N
    Grisham, M B
    Role of the proteasome in indomethacin-induced gastropathy1999In: Gastroenterology, Vol. 116, p. 865-873Article in journal (Refereed)
  • 338. Brand, S J
    et al.
    Tågerud, Sven
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lambert, P
    Magil, S G
    Tatarkiewicz, K
    Doiron, K
    Yan, Y
    Pharmacological treatment of chronic diabetes by stimulating pancreatic beta-cell regeneration with systemic co-administration of EGF and gastrin2002In: Pharmacology & Toxicology, Vol. 91, p. 414-420Article in journal (Refereed)
  • 339.
    Brandbyge, Mads
    et al.
    Tech Univ Denmark.
    Frederiksen, Thomas
    DIPC, San Sebastian 20018, Spain.
    Paulsson, Magnus
    University of Kalmar, School of Pure and Applied Natural Sciences.
    DFT-NEGF approach to current-induced forces, vibrational signals, and heating in nanoconductors2009In: Current-Driven Phenomena in Nanoelectronics / [ed] T. Seideman, Pan Stanford Publishing, 2009, p. 90-122Chapter in book (Other academic)
  • 340. Brandbyge, Mads
    et al.
    Frederiksen, Thomas
    Paulsson, Magnus
    University of Kalmar, School of Pure and Applied Natural Sciences.
    First-principles NEGF-DFT transport2006In: Report on multiscale approaches to modelling for nanotechnology / [ed] M. Macucci, S. Roche, A. Correia, 2006Chapter in book (Other academic)
  • 341. Bratt, T
    et al.
    Ohlson, Sten
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Borregaard, N
    Interactions between neutrophil gelatinase-associated lipocalin and natural hydrophobic ligands1999In: Biochimica et biophysica acta, Vol. 1472, p. 262-266Article in journal (Refereed)
  • 342. Braun, P
    et al.
    Persson, Bengt L.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Kaback, H R
    von Heijne, G
    Alanine insertion scanning mutagenesis of lactose permease transmembrane helices1997In: Journal of biological chemistry, Vol. 272, p. 29566-29571Article in journal (Refereed)
  • 343.
    Brodelius, Maria
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Hiraiwa, M
    Martilla, S
    Picaud, S
    Al-Karadaghi, S
    Brodelius, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Immunolocalization of the saposin-like insert of plant aspartic proteinases exhibiting saposin C activity in seeds and young flower tissues2005In: Physiologia Plantarum, Vol. 125, p. 405-418Article in journal (Refereed)
  • 344.
    Brodelius, Maria
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Lundgren, Anneli
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Mercke, Per
    Brodelius, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum2002In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 269, p. 3570-3577Article in journal (Refereed)
  • 345.
    Brodelius, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    High-performance Liquid Chromatographic Analysis of Analogous Amino and Oxo Acids for the Determination of Amino Acid Oxidase and Transaminase Activities1984In: Acta chemica Scandinavica, Series B - Organic Chemistry and Biochemistry, Vol. 38, no 3, p. 219-223Article in journal (Refereed)
  • 346.
    Brodelius, Peter
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Phenylpropanoid Metabolism in Vanilla planifolia Andr. V. Formation of Flavour Compounds in Developing Fruits1994In: Phytochemical analysis, Vol. 5, p. 27-31Article in journal (Refereed)
  • 347.
    Brodelius, Peter
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Mosbach, K
    The Utilization of Immobilized Substrate/Product in Affinity Chromatography. A Model Study using alfa-Chymotrypsin1973In: Acta chemica Scandinavica, Vol. 27, p. 2634-2638Article in journal (Refereed)
  • 348.
    Brodelius, Peter
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Mosbach, Klaus
    Separation of the Isoenzymes of Lactate Dehydrogenase by Affinity Chromatography Using an Immobilized AMP-Analogue1973In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 35, no 2, p. 223-226Article in journal (Refereed)
  • 349.
    Brodelius, Peter
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Nilsson, K
    Entrapment of Plant Cells in Different Matrices. A Comparative Study1980In: FEBS letters, Vol. 122, no 2, p. 312-316Article in journal (Refereed)
  • 350.
    Brodelius, Peter
    et al.
    University of Kalmar, School of Pure and Applied Natural Sciences.
    Pedersen, H
    Increasing Secondary Metabolite Production in Plant Cell Cultures by Redirecting Transport1993In: Trends in biotechnology, Vol. 11, p. 30-36Article in journal (Refereed)
45678910 301 - 350 of 2787
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