Burns injury can be caused by flame, heat, chemical and electricity and it remains as one of the most serious problems in the medical world. Burns injury can be treated with traditional medicine. One of them is Sembur Karo. The objective of this research was to investigate the Sembur Karo potency for burns injury treatment. Sembur Karo obtained from North Sumatera was identified its plant composition and analyzed for bioactive compounds; flavonoid, steroid, and tannin. Twenty five rats (Rattus norvegicus) were used. The rats were divided into five groups of five. The first group used as a control without treatment, the second group are sprayed with Sembur Karo, while the third and the fourth groups were applied with Sembur Karo added with water and human saliva respectively. The last group were treated with Bioplacentone. All treatments were done once a day for 3 wk. Each week, one rat from all group were sacrificed with skins were taken for histology specimen. From this research, it was concluded that bioactive compounds in Sembur Karo were isoquersetin, sterol, and condensed tannin. The skin histology showed that the group which sprayed with Sembur Karo had the most effective effect among all groups.