Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay 2021 (Engelska)Ingår i: The Complement System: Innovative Diagnostic and Research Protocols / [ed] Lubka T. Roumenina, Springer, 2021, Vol. 2227, s. 51-59Kapitel i bok, del av antologi (Refereegranskat)

Abstract [en]

Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.

Ort, förlag, år, upplaga, sidor

Springer, 2021. Vol. 2227, s. 51-59

Serie

Methods in Molecular Biology, ISSN 1940-6029, E-ISSN 1064-3745 ; 2227

Nyckelord [en]

2, 2’ azinobis(3 ethylbenzothiazoline 6 sulfonic acid), acetic acid, antibody, cobrotoxin, complement component C3a, edetic acid, hydrogen peroxide, polysorbate 20, zymosan, complement component C3a, epitope, monoclonal antibody, animal experiment, animal model, antibody specificity, coinfection, complement activation, controlled study, cross reaction, enzyme linked immunosorbent assay, human, in vivo study, nonhuman, pig, sepsis, animal, blood, complement activation, enzyme linked immunosorbent assay, goat, immunology, metabolism, mouse, physiology, pig, procedures, sepsis, swine disease, veterinary medicine, Animals, Antibodies, Monoclonal, Antibody Specificity, Complement Activation, Complement C3a, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Epitopes, Goats, Mice, Sepsis, Swine, Swine Diseases

Nationell ämneskategori

Immunologi

Forskningsämne

Biomedicinsk vetenskap, Immunologi

Identifikatorer

URN: urn:nbn:se:lnu:diva-110766DOI: 10.1007/978-1-0716-1016-9_5ISI: 000679413000006Scopus ID: 2-s2.0-85104313646Lokalt ID: 2021ISBN: 978-1-0716-1016-9 (digital)ISBN: 978-1-0716-1018-3 (tryckt)ISBN: 978-1-0716-1015-2 (tryckt)OAI: oai:DiVA.org:lnu-110766DiVA, id: diva2:1643665

Tillgänglig från: 2022-03-10 Skapad: 2022-03-10 Senast uppdaterad: 2025-09-23Bibliografiskt granskad

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Person

Nilsson, Per H.

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Av författaren/redaktören

Nilsson, Per H.

Av organisationen

Institutionen för kemi och biomedicin (KOB)Avancerade material

I ämnet

Immunologi

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