This study presents a comprehensive investigation of the dynamic behavior of a six-story lightweight timber frame building through periodic and continuous ambient vibration tests. Seasonal changes in temperature and relative humidity can influence the dynamic response of timber buildings, by affecting stiffness, strength, and connection properties due to changes in moisture content. Systematic tests were performed from October 2022 until May 2024 to identify natural frequencies, damping ratios, and mode shapes of the building using five battery-driven data acquisition units equipped with 15 uni-axialaccelerometers. Two different only-output frequency and time domain Operational Modal Analysis (OMA) methods were used to evaluate the dynamic properties of the building. Additionally, the building has been continuously monitored with temperature and humidity sensors since its construction. Results obtained from the periodic measurements highlighted the need for a permanent system to capture the transient changes in the modal parameters. A complementary permanent system to record the accelerations at the roof level was installed in May 2024. The modal parameters from in situ measurements were compared with those obtained from the Finite Element (FE) model of the structure. The FE model, calibrated through a multi-stage approach, incorporating non-structural elements, and varying imposed loads, showed good agreement with experimental data. Comparative analysis of the results obtained under different temperature and humidity conditions showed the effect of the environmental conditions on the building dynamics properties, for both periodic and permanent measurements. This study highlights the importance of continuous monitoring and detailed FE modeling in understanding the dynamic properties and long-term structural behavior under varying conditions. This information can be used to ensure that the building meets safety and performance requirements and to identify potential issues that may need to be addressed during the design and maintenance phases.