Zoos have important roles in society such as conservation, but also a responsibility concerning their captive animals’ welfare. One way of improving animal welfare is by providing enrichment and one such enrichment is the innovative animal exploration trails in 360 Zoo exhibits. These are supposed to enable the animals to travel larger, continuous distances and provide enriching visual, scent, and noise stimuli in hopes that the animals will have an activity budget closer to in their wild counterparts and having a larger behaviour repertoire. These are both ways to determine if animals’ welfare is good. However, these animal trails have yet to be evaluated for their effect on animal welfare, and this study aimed to evaluate how much a group of tufted capuchins (Cebus apella) at Öland Zoo and Amusement Park in Sweden used their animal trail and to investigate if their use was affected by the weather, temperature, or by visitors being present. The results show that the capuchins used the trail to a great extent, and that their use varied significantly depending on the weather, temperature and visitors. The data sample was, however questionable for these environmental factors and no strong associations were found for their effect, which was attributed to the capuchins possibly having a preference for being outside despite aversive conditions, but also to the design of the trail. Its design was maybe not optimal as it only had one entrance, which could impede its usefulness as a result of social factors for example. The trail does, however grant the capuchins a more complex environment, which is known to be related to better animal welfare, and the trail also gives the animals choice and control of their environment, which has been found to enable animals to better cope with stress and the presence of visitors. In conclusion, the animal trail was found to be a great extent by the capuchins and the trail could potentially provide better animal welfare, but the effect would depend on its design. Further research on animal trails’ effect of animal welfare is needed before expanding the its use.