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Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay
Linnaeus University, Faculty of Health and Life Sciences, Department of Chemistry and Biomedical Sciences. Linnaeus University, Linnaeus Knowledge Environments, Advanced Materials. University of Oslo, Norway;Oslo University Hospital Rikshospitalet, Norway. (Lnuc BMC;HoRB)ORCID iD: 0000-0002-7192-5794
Nordland Hospital, Norway.
Georg-August-University Göttingen, Germany.
Nordland Hospital, Norway;UiT—The Arctic University of Norway, Norway.
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2021 (English)In: The Complement System: Innovative Diagnostic and Research Protocols / [ed] Lubka T. Roumenina, Springer, 2021, Vol. 2227, p. 51-59Chapter in book (Refereed)
Abstract [en]

Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.
Place, publisher, year, edition, pages
Springer, 2021. Vol. 2227, p. 51-59
Series
Methods in Molecular Biology, ISSN 1940-6029, E-ISSN 1064-3745 ; 2227
Keywords [en]
2, 2’ azinobis(3 ethylbenzothiazoline 6 sulfonic acid), acetic acid, antibody, cobrotoxin, complement component C3a, edetic acid, hydrogen peroxide, polysorbate 20, zymosan, complement component C3a, epitope, monoclonal antibody, animal experiment, animal model, antibody specificity, coinfection, complement activation, controlled study, cross reaction, enzyme linked immunosorbent assay, human, in vivo study, nonhuman, pig, sepsis, animal, blood, complement activation, enzyme linked immunosorbent assay, goat, immunology, metabolism, mouse, physiology, pig, procedures, sepsis, swine disease, veterinary medicine, Animals, Antibodies, Monoclonal, Antibody Specificity, Complement Activation, Complement C3a, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Epitopes, Goats, Mice, Sepsis, Swine, Swine Diseases
National Category
Immunology
Research subject
Biomedical Sciences, Immunology
Identifiers
URN: urn:nbn:se:lnu:diva-110766DOI: 10.1007/978-1-0716-1016-9_5ISI: 000679413000006Scopus ID: 2-s2.0-85104313646Local ID: 2021ISBN: 978-1-0716-1016-9 (electronic)ISBN: 978-1-0716-1018-3 (print)ISBN: 978-1-0716-1015-2 (print)OAI: oai:DiVA.org:lnu-110766DiVA, id: diva2:1643665
Available from: 2022-03-10 Created: 2022-03-10 Last updated: 2025-09-23Bibliographically approved

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Nilsson, Per H.

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