The main purpose of this study is to develop a SPME calibration method suitable for use in evaluation of concentrations of hydrophobic substances

in environmental samples.

The analyte used in the experiments was alpha-pinene, a hydrophobic organic compound commonly found in wood, and therefore found in

wood storage facilities, wood processing industries and wood based biofilters. The SPME fibres were calibrated for different concentrations of

alpha-pinene at different temperatures and relative humidities. The method was used to evaluate the removal efficiency of a lab-scale biofilter.