Azo dye degradation occurs by means of the oxidation–reduction reactions which have the azo dye acting as final electron acceptor. Some carbon sources can act as electron donors because the products of their metabolism act as redox mediators. In order to enhance the dye biodegradation process, the present study aims to evaluate the decolorization of an artificial wastewater, containing the azo dye Direct Red 75 (DR75), led by a microbial consortium from rice husks, testing the effect in the process of glucose and yeast extract as carbon sources. Samples with and without 0.1 M Sodium phosphate buffer were also analysed. The decolorization was measured by means UV-VIS spectrophotometry. The percentage of decolorization of the samples over the time indicates that the sample with yeast extract, rinse water of rice husks and without buffer presented the best decolorization rate, about 80%. Therefore, the results presented in this study may also suggest that yeast extract is a better carbon source for dye biodegradation than glucose.